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Detailed Vector Information: pBY011


Vector Name: pBY011
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Synonyms: ''


Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp): 8121
Empty Vector: None
Parent Vector: Name: YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Properties: Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Bhullar
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3651 4333
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 4915 5124
primer M13 forward M13 forward sequencing primer 7183 7200
primer M13 reverse M13 reverse sequencing primer 4702 4722
promoter Gal1 Gal1-10 yeast promoter 6934 7145
promoter Ura3 promoter URA3 promoter 2349 2378
recombination site attR1 attR recombination site 1, lost or altered in recombined (with insert) form 6439 6455
recombination site attR2 attR recombination site 2, lost or altered in recombined (with insert) form 4749 4764
selectable marker AmpR ampicillin resistance gene 2894 3553
selectable marker CmR chloramphenicol resistance gene LOST in recombined (with insert) form 5566 6222
selectable marker Ura3 URA3 1460 2263
yeast centromere CEN yeast centromeric sequence 255 7396
yeast origin ARS1 ARS1 yeast origin of replication 0 0