Detailed Vector Information: pBY011

Vector Name:
pBY011
Synonyms:
''
Sequencing Primer:
Forward:  M13F
Reverse:  M13R
Description:
Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Comments:
This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.
Size (bp):
8121
Parent Vector:
Name: YCplac33
Description: Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.
Properties:
Gateway, acceptor (destination), inducible expression, recombinational cloning, with tag/fusion/marker, yeast expression, yeast shuttle
Author Name:
Bhullar
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3651
4333
negative selection marker
ccdB
ccdB negative selection marker gene LOST in recombined (with insert) form
4915
5124
primer
M13 forward
M13 forward sequencing primer
7183
7200
primer
M13 reverse
M13 reverse sequencing primer
4702
4722
promoter
Gal1
Gal1-10 yeast promoter
6934
7145
promoter
Ura3 promoter
URA3 promoter
2349
2378
recombination site
attR1
attR recombination site 1, lost or altered in recombined (with insert) form
6439
6455
recombination site
attR2
attR recombination site 2, lost or altered in recombined (with insert) form
4749
4764
selectable marker
AmpR
ampicillin resistance gene
2894
3553
selectable marker
CmR
chloramphenicol resistance gene LOST in recombined (with insert) form
5566
6222
selectable marker
Ura3
URA3
1460
2263
yeast centromere
CEN
yeast centromeric sequence
255
7396
yeast origin
ARS1
ARS1 yeast origin of replication
0
0