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Detailed Vector Information: pDB.TRX


Vector Name: pDB.TRX
       DyNA Vector Map

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Synonyms: pTRX, pX


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector that adds thioredoxin and TEV protease sites; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5693
Empty Vector: EvNO00085133
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin f1 F1 origin 29 335
bacterial origin ColE ColE1 origin 1421 2103
promoter T7 T7 promoter 4984 5003
protease cleavage site TEV TEV protease cleavage site cds 5431 5448
repressor binding site lac operator lac operator 5003 5025
repressor protein gene LacI LacI gene 2515 4594
selectable marker KanR kanamycin resistance gene 563 1375
tag TRX thioredoxin cds tag 5080 5403
trxn termination sequence T7 term T7 term sequencing primer 5610 5628