Vector Name:
pDB-HisGST
Synonyms:
pDB.His.GST, pHG
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
6053
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Berkeley Structural Genomics Center
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1421
2103
promoter
T7
T7 promoter
4984
5003
protease cleavage site
TEV
TEV protease cleavage site
5791
5808
repressor binding site
lacO
lacO sequence region
5003
5025
repressor protein gene
lacI
lacI coding region
3518
4597
selectable marker
KanR
kanamycin resistance gene
1375
563
ssDNA origin
f1 ori
f1 origin
29
335
tag
His
6xHis tag
5083
5100
tag
GST
GST tag
5104
5771
trxn termination sequence
T7 term
T7 termination sequence
5982
6028
