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Detailed Vector Information: pDB-HisGST

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Vector Name: pDB-HisGST
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Synonyms: pDB.His.GST, pHG

pDB-HisGST

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector that adds N-terminal His6 tag, GST, TEV protease site; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 6053
Empty Vector: EvNO00085134
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Berkeley Structural Genomics Center
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 1421 2103
promoter T7 T7 promoter 4984 5003
protease cleavage site TEV TEV protease cleavage site 5791 5808
repressor binding site lacO lacO sequence region 5003 5025
repressor protein gene lacI lacI coding region 3518 4597
selectable marker KanR kanamycin resistance gene 1375 563
ssDNA origin f1 ori f1 origin 29 335
tag GST GST tag 5104 5771
tag His 6xHis tag 5083 5100
trxn termination sequence T7 term T7 termination sequence 5982 6028