Detailed Vector Information: pET28a

Vector Name:
pET28a
Synonyms:
pET-28a, pET28
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7lac promoter, adds N-terminal His tag, thrombin cleavage site, internal T7 epitope tag, C-terminal His tag; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5369
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
MCS
MCS
MCS (XhoI, NotI, EagI, HindIII, SalI, SacI, EcoRI, BamHI, NheI, NdeI, NcoI)
158
203
bacterial origin
f1
f1 origin
4903
5358
bacterial origin
ori
bacterial origin of replication
3270
3889
promoter
T7
T7 promoter
368
386
repressor protein gene
LacI
lacI coding sequence (lacI cds)
896
1852
selectable marker
KanR
kanamycin resistance
3995
4807
tag
His
6xHis tag
140
157
trxn regulatory element
lac operator
lac operator
347
363
trxn termination sequence
T7 term
T7 terminator
26
72