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Detailed Vector Information: pET28-N-His-thromb

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Vector Name: pET28-N-His-thromb
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Synonyms: N-His-Thrombin pET28

pET28-N-His-thromb

Sequencing Primer: Forward:  T7
Reverse:  ISFI_newrev
Description: Expression vector with T7 promoter, adds N-terminal His tag, thrombin site; kanamycin resistance; restriction enzyme cloning.
Comments: None
Size (bp): 5369
Empty Vector: None
Parent Vector: None
Properties: cell-free expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Integrated Center for Structure and Function Innovation
Publications: PMID: 16374842
Title: Crystal structure of a putative pyridoxine 5'-phosphate oxidase (Rv2607) from Mycobacterium tuberculosis

PMID: 16885468
Title: Solution structure of the conserved hypothetical protein Rv2302 from Mycobacterium tuberculosis

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 1421 2103
primer reverse reverse sequencing primer 5?-TTTGTTAGCAGCCGGATCTCAGTGG-3? 5861 5885
promoter T7 T7 promoter 4987 5006
protease cleavage site Thrombin N-terminal His/thrombin cleavage site 0 0
repressor protein gene LacI LacI 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His N-terminal 6xHis tag 5847 5864
trxn regulatory element lac operator lac operator 5006 5028