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Detailed Vector Information: pEU-GST-FV


Vector Name: pEU-GST-FV
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Synonyms: ''


Sequencing Primer: Forward:  GST Forward
Reverse:  pEU Reverse
Description: Cell-free expression vector, adds N-terminal GST tag and 3CP cleavage site; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 5612
Empty Vector: EvNO00084284
Parent Vector: None
Properties: cell-free expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15782178
Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3081 3763
gene fragment Omega Omega 25 148
gene fragment tet operator TMV-L 2466 2526
gene fragment Barnase BarCat cassette, including Barnase 863 1199
gene fragment CAT BarCat cassette, including CAT 1301 1958
promoter SP6 SP6 promoter 1 18
protease cleavage site 3C 3C cleavage site cds 812 835
recombination site MCS pF1K (Flexi-Vector) homology domain 1966 2094
selectable marker AmpR ampicillin resistance gene 3861 4520
tag GST GST coding sequence (GST cds) 149 805
trxn termination sequence T7 term T7 terminator sequence 2075 2094