Detailed Vector Information: pMCSG10

Vector Name:
pMCSG10
Synonyms:
pMCSG-10
Sequencing Primer:
Forward:  T7short
Reverse:  T7 terminator
Description:
with tag/fusion/marker assay, multiple cloning site clone, bacterial expression, improves solubility of insert expression with lacI and LIC and AmpR and T7 and T7 term and ori and TEV and His and His 2 and GST; ampicillin resistance in bacterial;
Comments:
pMCSG7 derivative
Size (bp):
5961
Parent Vector:
None
Properties:
bacterial expression, improves solubility of insert, multiple cloning site, with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
Powered by LabGenius

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
ligation independent cloning (LIC) site
204
235
bacterial origin
ori
ColE1-type origin of replication
3955
3955
promoter
T7
T7 promoter sequence
1035
1051
protease cleavage site
TEV
TEV protease cleavage site
223
243
repressor protein gene
lacI
lacI coding sequence
1565
2524
selectable marker
AmpR
ampicillin resistance gene
4713
5573
tag
His
N-terminal 6xHis tag
943
960
tag
GST
GST tag
265
924
tag
His 2
6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72