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Detailed Vector Information: pMCSG11


Vector Name: pMCSG11
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Synonyms: pMCSG-11


Sequencing Primer: Forward:  DuetUp1
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4079
Empty Vector: EvNO00084259
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 353 384
MCS MCS MCS (NcoI, BamHI, EcoRI, PstI, HindIII) 1801 1880
MCS MCS2 MCS (BamHI, EcoRI, HindIII, AvaI) 2171 2216
bacterial origin ori P15A-type bacterial origin of replication 1821 2733
primer site Duet Down1 Duet DOWN1 primer site 189 208
primer site Duet P1 ACYC Duet U P1 primer site 4020 4039
primer site Duet P2 Duet P2 primter site 189 208
promoter T7 T7 promoter 214 230
promoter T7 2 T7 promoter 4063 4079
protease cleavage site TEV TEV protease cleavage site 345 365
repressor binding site lacO1 lacO sequence region 5 25
repressor binding site lacO2 lacO sequence region 235 230
repressor protein gene lacI lacI cds 2857 3939
ribosome binding site RBS1 ribosome binding site 58 63
ribosome binding site RBS2 ribosome binding site 286 291
selectable marker CmR chloramphenicol resistance gene (CAT) 800 1459
tag His N-terminal His tag 83 100
tag S-tag N-terminal S-tag 437 481
tag His 2 6xHis tag 303 320
trxn termination sequence T7 term T7 termination sequence 533 580