Detailed Vector Information: pMCSG12

Vector Name:
pMCSG12
Synonyms:
pMCSG-12
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments:
pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
4133
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
407
438
bacterial origin
ori
P15A-type bacterial origin of replication
1875
2787
gene fragment
CAT
CAT coding sequence
854
1513
promoter
T7
T7 promoter
214
230
promoter
T7
T7 promoter (upstream of another one)
4117
4133
protease cleavage site
TEV
TEV cleavage site cds
399
419
repressor protein gene
LacI
lacI coding sequence
2911
3993
ribosome binding site
RBS2
Ribosome binding site
286
291
ribosome binding site
RBS
RBS-1: ribosome binding site
58
63
tag
His
N-terminal 6xHis tag
83
100
tag
His 2
6xHis tag
303
320
tag
S loop
N-terminal S loop
339
392
trxn regulatory element
lac operator
lac operator sequence
5
25
trxn termination sequence
T7 term
T7 terminator sequence
587
634