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Detailed Vector Information: pMCSG13


Vector Name: pMCSG13
       DyNA Vector Map

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Synonyms: pMCSG-13


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments: pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 5198
Empty Vector: EvNO00084261
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 1472 1503
bacterial origin ori P15A-type bacterial origin of replication 2940 3852
gene fragment CAT CAT coding sequence 1919 2578
promoter T7 T7 promoter 5182 5198
protease cleavage site TEV TEV cleavage site cds 1464 1484
repressor binding site lac operator LacO fragment 5 25
repressor binding site lac operator 2 LacO fragment 235 255
repressor protein gene LacI LacI coding region 3976 5058
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
tag MBP Maltose Binding Protein cds 345 1442
tag His 6xHis tag 83 100
tag His 2 6xHis tag 303 320
tag S-tag S-tag 1556 1600
trxn termination sequence T7 term T7 terminator sequence 1652 1699