Vector Name:
pMCSG13
Synonyms:
pMCSG-13
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; chloramphenicol resistance; ligation independent cloning (LIC).
Comments:
pACYCDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
5198
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID:
18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
1472
1503
bacterial origin
ori
P15A-type bacterial origin of replication
2940
3852
gene fragment
CAT
CAT coding sequence
1919
2578
promoter
T7
T7 promoter
5182
5198
protease cleavage site
TEV
TEV cleavage site cds
1464
1484
repressor binding site
lac operator
LacO fragment
5
25
repressor binding site
lac operator 2
LacO fragment
235
255
repressor protein gene
LacI
LacI coding region
3976
5058
ribosome binding site
RBS
Ribosome binding site
58
63
ribosome binding site
RBS2
Ribosome binding site
286
291
tag
MBP
Maltose Binding Protein cds
345
1442
tag
His
6xHis tag
83
100
tag
His 2
6xHis tag
303
320
tag
S-tag
S-tag
1556
1600
trxn termination sequence
T7 term
T7 terminator sequence
1652
1699
