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Detailed Vector Information: pMCSG21

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Vector Name: pMCSG21
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Synonyms: pMCSG-21

pMCSG21

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 3852
Empty Vector: EvNO00084267
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning region 353 384
MCS MCS MCS (BamHI, EcoRI, HindIII, AvaI) 385 430
MCS MCS MCS (BamHI, EcoRI, PstI, HindIII) 106 148
bacterial origin ori CloDF13-type bacterial origin of replication 1682 2420
promoter T7 T7 promoter 214 230
protease cleavage site TEV TEV cleavage site cds 345 365
repressor binding site lac operator lacO seqeuence region 5 25
repressor binding site lac operator 2 lacO sequence region 235 255
repressor protein gene LacI lacI cds 2630 3589
ribosome binding site RBS2 ribosome binding site 286 291
ribosome binding site RBS 3 Ribosome binding site 1548 1552
ribosome binding site RBS ribosome binding site 58 63
selectable marker SpectR spectinomycin resistance gene aadA 751 1542
tag His 2 6xHis tag 303 320
tag His 6xHis tag 83 100