Detailed Vector Information: pMCSG21

Vector Name:
pMCSG21
Synonyms:
pMCSG-21
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments:
pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
3852
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 17363272
Title: New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning region
353
384
MCS
MCS
MCS (BamHI, EcoRI, HindIII, AvaI)
385
430
MCS
MCS
MCS (BamHI, EcoRI, PstI, HindIII)
106
148
bacterial origin
ori
CloDF13-type bacterial origin of replication
1682
2420
promoter
T7
T7 promoter
214
230
protease cleavage site
TEV
TEV cleavage site cds
345
365
repressor binding site
lac operator
lacO seqeuence region
5
25
repressor binding site
lac operator 2
lacO sequence region
235
255
repressor protein gene
LacI
lacI cds
2630
3589
ribosome binding site
RBS
ribosome binding site
58
63
ribosome binding site
RBS2
ribosome binding site
286
291
ribosome binding site
RBS 3
Ribosome binding site
1548
1552
selectable marker
SpectR
spectinomycin resistance gene aadA
751
1542
tag
His
6xHis tag
83
100
tag
His 2
6xHis tag
303
320