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Detailed Vector Information: pMCSG22

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Vector Name: pMCSG22
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Synonyms: pMCSG-22

pMCSG22

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, Sloop, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 3906
Empty Vector: EvNO00084268
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 407 438
bacterial origin ori CloDF-type bacterial origin of replication 1736 2474
promoter T7 2 T7 promoter 3890 3906
promoter T7 T7 promoter 3890 3906
promoter T7 T7 promoter (2) 214 230
protease cleavage site TEV TEV cleavage site cds 399 419
repressor binding site lac operator 2 LacO fragment 235 255
repressor binding site lac operator LacO fragment 5 25
repressor protein gene LacI LacI coding sequence 2684 3643
ribosome binding site RBS 3 Ribosome binding site 1602 1606
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
selectable marker SpectR Spectinomycin resistance gene (aadA) 805 1596
tag His 6xHis tag 83 100
tag His 2 6xHis tag 303 320
tag S loop S loop 339 392
trxn termination sequence T7 term T7 terminator sequence 587 634