Vector Name:
pMCSG23
Synonyms:
pMCSG-23
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His, MBP, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments:
pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
4971
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID:
18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
1472
1503
bacterial origin
ori
CloDF bacterial origin of replication
2801
3539
promoter
T7
T7 promoter
4955
4971
promoter
T7 2
T7 promoter
214
230
protease cleavage site
TEV
TEV cleavage site cds
1464
1484
repressor binding site
lac operator
LacO fragment
5
25
repressor binding site
lac operator 2
LacO fragment
235
255
repressor protein gene
LacI
LacI coding sequence
3749
4708
ribosome binding site
RBS
Ribosome binding site
58
63
ribosome binding site
RBS2
Ribosome binding site
286
291
ribosome binding site
RBS 3
Ribosome binding site
2667
2671
selectable marker
SpectR
Spectinomycin resistance gene
1870
2661
tag
MBP
Maltose Binding Protein cds
345
1442
tag
His
6xHis tag
83
100
tag
His 2
6xHis tag
303
320
trxn termination sequence
T7 term
T7 terminator sequence
1652
1699
