Detailed Vector Information: pMCSG24

Vector Name:
pMCSG24
Synonyms:
pMCSG-24
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments:
pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp):
4527
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
Powered by LabGenius

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
1028
1059
bacterial origin
ori
CloDF-type origin of replication
2357
3095
promoter
T7
T7 promoter
4511
4527
promoter
T7 2
T7 promoter
214
230
protease cleavage site
TEV
TEV cleavage site cds
1020
1040
repressor binding site
LacI
LacI coding sequence
3305
4264
repressor binding site
lac operator
LacO fragment
5
25
repressor binding site
lac operator 2
LacO fragment
235
255
ribosome binding site
RBS
Ribosome binding site
58
63
ribosome binding site
RBS2
Ribosome binding site
286
291
ribosome binding site
RBS 3
Ribosome binding site
2223
2227
selectable marker
SpectR
Spectinomycin resistance gene
1426
2217
tag
His
6xHis tag
83
100
tag
His 2
6xHis tag
303
320
tag
GST
GST tag
339
998
trxn termination sequence
T7 term
T7 terminator sequence
1208
1255