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Detailed Vector Information: pMCSG24

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Vector Name: pMCSG24
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Synonyms: pMCSG-24

pMCSG24

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Coexpression vector with T7 promoter, adds N-terminal His, GST, TEV, SNA; spectinomycin resistance; ligation independent cloning (LIC).
Comments: pCDFDuet-1 derivative, 2nd region replaced with LIC region
Size (bp): 4527
Empty Vector: EvNO00084270
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning (LIC) site 1028 1059
bacterial origin ori CloDF-type origin of replication 2357 3095
promoter T7 2 T7 promoter 214 230
promoter T7 T7 promoter 4511 4527
protease cleavage site TEV TEV cleavage site cds 1020 1040
repressor binding site lac operator 2 LacO fragment 235 255
repressor binding site lac operator LacO fragment 5 25
repressor binding site LacI LacI coding sequence 3305 4264
ribosome binding site RBS 3 Ribosome binding site 2223 2227
ribosome binding site RBS Ribosome binding site 58 63
ribosome binding site RBS2 Ribosome binding site 286 291
selectable marker SpectR Spectinomycin resistance gene 1426 2217
tag His 6xHis tag 83 100
tag His 2 6xHis tag 303 320
tag GST GST tag 339 998
trxn termination sequence T7 term T7 terminator sequence 1208 1255