Detailed Vector Information: pMCSG8

Vector Name:
pMCSG8
Synonyms:
pMCSG-8
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative; Sloop = loop of GroES that binds GroEL
Size (bp):
5340
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
PMID: 11483004
Title: Expression of a highly toxic protein, Bax, in Escherichia coli by attachment of a leader peptide derived from the GroES cochaperone
PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning site
204
235
bacterial origin
ori
ColE1-type bacterial origin of replication
3997
3315
promoter
T7
T7 promoter
414
430
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
389
411
repressor protein gene
LacI
LacI coding region
944
1903
selectable marker
AmpR
ampicillin resistance gene
4092
4952
tag
His
N-terminal 6x His tag
322
339
tag
His 2
C-terminal 6x His tag
140
157
tag
S loop
N-terminal S loop
250
302
trxn termination sequence
T7 term
T7 terminator sequence
26
72