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Detailed Vector Information: pMCSG8


Vector Name: pMCSG8
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Synonyms: pMCSG-8


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector facillitating reduced protein toxicity, with T7 promoter, adds N-terminal His tag, Sloop, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative; Sloop = loop of GroES that binds GroEL
Size (bp): 5340
Empty Vector: EvNO00084272
Parent Vector: None
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 11483004
Title: Expression of a highly toxic protein, Bax, in Escherichia coli by attachment of a leader peptide derived from the GroES cochaperone

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS LIC Ligation independent cloning site 204 235
bacterial origin ori ColE1-type bacterial origin of replication 3997 3315
promoter T7 T7 promoter 414 430
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 389 411
repressor protein gene LacI LacI coding region 944 1903
selectable marker AmpR ampicillin resistance gene 4092 4952
tag His N-terminal 6x His tag 322 339
tag His 2 C-terminal 6x His tag 140 157
tag S loop N-terminal S loop 250 302
trxn termination sequence T7 term T7 terminator sequence 26 72