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Detailed Vector Information: pMCSG9


Vector Name: pMCSG9
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Synonyms: pMCSG-9


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Vector with tags for solubility of insert, with T7 promoter, adds N-terminal His tag, MBP, TEV, SNA; amp resistance; ligation independent cloning (LIC).
Comments: pMCSG7 derivative
Size (bp): 6405
Empty Vector: EvNO00084273
Parent Vector: None
Properties: bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 16497515
Title: An expression vector tailored for large-scale, high-throughput purification of recombinant proteins

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) site 204 235
bacterial origin ori pBR322-type bacterial origin of replication 4399 4399
promoter T7 T7 promoter 1479 1495
protease cleavage site TEV TEV protease cleavage site cds 223 243
repressor binding site lac operator lacO regulatory sequence 1454 1476
repressor protein gene LacI LacI cds 2009 2968
selectable marker AmpR ampicillin resistance 5157 6017
tag His 6xHis tag 140 157
tag His 2 N-terminal 6xHis tag 1387 1404
tag MBP maltose binding protein cds 265 1362
trxn termination sequence T7 term T7 terminator 26 72