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Detailed Vector Information: pMH2T7

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Vector Name: pMH2T7
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Synonyms: pMH-2-T7

pMH2T7

Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial (E. coli) expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enzyme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 4084
Empty Vector: None
Parent Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColEI origin of replication 1921 2603
promoter T7 T7 promoter 290 310
promoter araC araC promoter 256 285
selectable marker AmpR ampicillin resistance gene 1164 1823
tag His N-terminal 6x His tag 367 381
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182