Detailed Vector Information: pSGP40

Vector Name:
pSGP40
Synonyms:
pSGP-40
Sequencing Primer:
Forward:  pSGP-Forward
Reverse:  pSGP-Reverse
Description:
Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
Comments:
None
Size (bp):
6564
Parent Vector:
None
Properties:
inducible expression, ligation independent cloning (LIC), with tag/fusion/marker, yeast expression, yeast shuttle
Author Name:
Center for High Throughput Structural Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
2499
3181
promoter
Ura3 promoter
Ura3 promoter
6249
6278
promoter
T7
T7 promoter
4588
4608
protease cleavage site
3C
3C protease cleavage site
4122
4145
reporter gene
LacZ
LacZ alpha gene (beta-galactosidase)
4705
4773
selectable marker
AmpR
ampicillin resistance gene
1742
2401
selectable marker
Ura3
URA3 nutritional marker
5360
6163
tag
His
10xHis tag (LIC-3C-ZZ-His10)
4542
4571
trxn regulatory element
lac operator
lac operator
3521
3543