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Detailed Vector Information: pSGP40


Vector Name: pSGP40
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Synonyms: pSGP-40


Sequencing Primer: Forward:  pSGP-Forward
Reverse:  pSGP-Reverse
Description: Yeast expression vector with Gal1 promoter, adds C-terminal 3C-ZZ-His10 tag; amp resistance (URA for yeast); ligation independent cloning (LIC).
Comments: None
Size (bp): 6564
Empty Vector: EvNO00304419
Parent Vector: None
Properties: inducible expression, ligation independent cloning (LIC), with tag/fusion/marker, yeast expression, yeast shuttle
Author Name: Center for High Throughput Structural Biology
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 2499 3181
promoter Ura3 promoter Ura3 promoter 6249 6278
promoter T7 T7 promoter 4588 4608
protease cleavage site 3C 3C protease cleavage site 4122 4145
reporter gene LacZ LacZ alpha gene (beta-galactosidase) 4705 4773
selectable marker AmpR ampicillin resistance gene 1742 2401
selectable marker Ura3 URA3 nutritional marker 5360 6163
tag His 10xHis tag (LIC-3C-ZZ-His10) 4542 4571
trxn regulatory element lac operator lac operator 3521 3543