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Detailed Vector Information: pVP13

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Vector Name: pVP13
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Synonyms: None

pVP13

Sequencing Primer: Forward:  MBP Forward
Reverse:  pQE Reverse
Description: Bacterial (E. coli) expression, adds N-terminal S-tag, 6xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments: TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp): 7675
Empty Vector: EvNO00084278
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6516 5834
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 2688 2897
promoter T5 T5 promoter 7 87
recombination site AttR1 AttR1 recombination site 1361 1485
recombination site AttR2 AttR2 recombination site 3065 2941
repressor binding site LacI LacI coding sequence 4177 5256
selectable marker CmR chloramphenicol resistance gene (CmR) (lost in with-insert form) 1594 2250
selectable marker AmpR ampicillin resistance gene 7273 6614
tag His N-terminal 6xHis tag 173 190
tag S-tag N-terminal S-tag 116 152
tag MBP N-terminal MBP tag 193 1291