Detailed Vector Information: pVP13

Vector Name:
pVP13
Synonyms:
''
Sequencing Primer:
Forward:  MBP Forward
Reverse:  pQE Reverse
Description:
Bacterial (E. coli) expression, adds N-terminal S-tag, 6xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments:
TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp):
7675
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID: 15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
6516
5834
negative selection marker
ccdB
ccdB death cassette gene (lost in with-insert form)
2688
2897
promoter
T5
T5 promoter
7
87
recombination site
AttR1
AttR1 recombination site
1361
1485
recombination site
AttR2
AttR2 recombination site
3065
2941
repressor binding site
LacI
LacI coding sequence
4177
5256
selectable marker
CmR
chloramphenicol resistance gene (CmR) (lost in with-insert form)
1594
2250
selectable marker
AmpR
ampicillin resistance gene
7273
6614
tag
S-tag
N-terminal S-tag
116
152
tag
MBP
N-terminal MBP tag
193
1291
tag
His
N-terminal 6xHis tag
173
190