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Detailed Vector Information: pVP33A

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Vector Name: pVP33A
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Synonyms: None

pVP33A

Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, tetra-cys, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 7228
Empty Vector: EvNO00084280
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori pBR322-type bacterial origin of replication 5422 6104
gene fragment BarCat-cat CAT cds - chloramphenicol resistance 1819 2476
gene fragment BarCat-bar Barnase cds 1381 1717
primer site T7 rev T7 reverse primer site 2533 2551
promoter T5 pr 2 T5 promoter (two of two) 0 0
promoter T5 T5 promoter 43 89
protease cleavage site TEV TEV cleavage site cds 1335 1355
protease cleavage site 3C 3CP protease cleavage site cds 1293 1317
recombination site pF1K site pF1K homology site 2484 2612
repressor protein gene LacIq LacIq 3762 4841
selectable marker AmpR ampicillin resistance gene 6202 6861
tag His 8xHis epitope tag 157 180
tag tetraCys tetraCyc cds 1318 1335
tag MBP MBP fusion protein tag 187 1283