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Detailed Vector Information: pVP33K

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Vector Name: pVP33K
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Synonyms: None

pVP33K

Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression, adds N-terminal 6xHis tag, MBP, 3CP cleavage, tetra-cys, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 7253
Empty Vector: EvNO00084285
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 5502 6184
promoter T5 T5 promoter 123 169
protease cleavage site 3C 3CP cleavage site 1373 1397
protease cleavage site TEV TEV protease cleavage site 1415 1435
repressor protein gene LacI LacI cds 3842 4858
selectable marker CmR chloramphenicol resistance gene (CmR) 1900 2556
tag MBP MBP tag 267 1363
tag His 6xHis tag 237 260