Vector Name:
pVP56A
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments:
Cloning into this vector will remove the BarCat casstte
Size (bp):
7222
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
6098
5416
promoter
T5
T5 promoter
43
89
protease cleavage site
3C
3C protease cleavage site
1308
1332
protease cleavage site
TEV
TEV protease cleavage site
1333
1353
repressor protein gene
LacI
LacI cds
3759
4838
selectable marker
CmR
chloramphenicol resistance gene (CmR)
1817
2473
selectable marker
Barnase
Barnase gene
1378
1714
selectable marker
AmpR
ampicillin resistance gene
6855
6196
tag
His
6xHis tag
157
180
tag
MBP
MBP tag
187
1283
