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Detailed Vector Information: pVP56A

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Vector Name: pVP56A
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Synonyms: None

pVP56A

Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; amp resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 7222
Empty Vector: EvNO00084281
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 6098 5416
promoter T5 T5 promoter 43 89
protease cleavage site TEV TEV protease cleavage site 1333 1353
protease cleavage site 3C 3C protease cleavage site 1308 1332
repressor protein gene LacI LacI cds 3759 4838
selectable marker Barnase Barnase gene 1378 1714
selectable marker AmpR ampicillin resistance gene 6855 6196
selectable marker CmR chloramphenicol resistance gene (CmR) 1817 2473
tag MBP MBP tag 187 1283
tag His 6xHis tag 157 180