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Detailed Vector Information: pVP56K


Vector Name: pVP56K
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Synonyms: ''


Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression, adds N-terminal 8xHis, MBP, 3CP cleavage, Tev cleavage; kanamycin resistance; restriction enzyme cloning (SgfI, PmeI).
Comments: Cloning into this vector will remove the BarCat casstte
Size (bp): 7253
Empty Vector: EvNO00084282
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: PMID: 16377204
Title: High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type bacterial origin of replication 5502 6184
promoter T5 T5 promoter 123 169
protease cleavage site TEV TEV protease cleavage site 1413 1433
protease cleavage site 3C 3C protease cleavage site 1388 1412
repressor protein gene LacI LacI cds 3845 4924
selectable marker Barnase Barnase gene 1464 1800
selectable marker CmR Chlorampenicol resistance gene (CAT) 1903 2556
tag His 8xHis tag 237 260
tag MBP MBP tag 267 1363