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Detailed Vector Information: pMCentr2

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Vector Name: pMCentr2
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Synonyms: pMCentr-2

pMCentr2

Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: cloning vector to link to Gateway system with attL1-TEV-LIC site-attL2; kanamycin resistance; ligation independent cloning to make pENTR vector for recombinational cloning
Comments: From pDONR221; LIC generates ENTR vector
Size (bp): 2586
Empty Vector: EvNO00084276
Parent Vector: None
Properties: Gateway, donor (entry), fluorescent marker, ligation independent cloning (LIC), mammalian expression, recombinational cloning, ssDNA production, with tag/fusion/marker
Author Name: M.Donnelly
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 0 0
ligation independent cloning LIC ligation independent cloning (LIC) 679 710
primer site M13 forward sequencing primer site 537 552
primer site M13 reverse M13 reverse sequencing primer 851 867
protease cleavage site TEV protease cleavage site 671 691
recombination site attL2 attL2 recombination site 711 810
recombination site attL1 attL1 recombination site 569 668
selectable marker KanR kanamycin resistance gene 980 1789
trxn termination sequence rrnB rrnB T1 and T2 transcription terminator 268 470