Vector Name:
pSGX5
Synonyms:
pSGX5
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, ULP-1 cleavage site; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen. Smt3 fusion at N-terminus. Clone using BamHI and either HindIII, XhoI or NotI
Size (bp):
5633
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
start codon
5071
5073
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
primer site
T7
T7 sequencing primers
4985
5000
promoter
T7
T7 promoter
4984
5003
protease cleavage site
ULP site
ULP cleavage site cds
5242
5242
repressor protein gene
LacI
LacI gene
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
Smt3
N-terminal Smt3 tag
5140
5242
tag
His
6xHis tag
5083
5100
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 termination sequence
5562
5608
