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Detailed Vector Information: pMCSG29


Vector Name: pMCSG29
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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp): 6389
Empty Vector: EvNO00287601
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4383 4383
promoter T7 T7 promoter 1463 1479
protease cleavage site TVMV TVMV protease cleavage site 1311 1331
protease cleavage site TEV TEV protease cleavage site 1368 1388
recombination site LIC Ligation Independent Cloning (LIC) recombination site 1382 1400
repressor binding site LacI LacI coding region 1993 2952
repressor binding site lac operator LacO fragment 3363 3385
selectable marker AmpR Ampicillin resistance gene 5141 6001
tag His C-terminal 10xHis Tag 1332 1361
tag MBP C-terminal Maltose binding protein (MBP) tag 204 1310
trxn termination sequence T7 term T7 transcription termination sequence 26 72