Vector Name:
pMCSG29
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, C-terminal 10xHis tag, TEV site, C-terminal MBP with TVMV site; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments:
For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp):
6389
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
Publications:
PMID:
20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
4383
4383
promoter
T7
T7 promoter
1463
1479
protease cleavage site
TVMV
TVMV protease cleavage site
1311
1331
protease cleavage site
TEV
TEV protease cleavage site
1368
1388
recombination site
LIC
Ligation Independent Cloning (LIC) recombination site
1382
1400
repressor binding site
LacI
LacI coding region
1993
2952
repressor binding site
lac operator
LacO fragment
3363
3385
selectable marker
AmpR
Ampicillin resistance gene
5141
6001
tag
His
C-terminal 10xHis Tag
1332
1361
tag
MBP
C-terminal Maltose binding protein (MBP) tag
204
1310
trxn termination sequence
T7 term
T7 transcription termination sequence
26
72
