Detailed Vector Information: pHM3C-LIC

Vector Name:
pHM3C-LIC
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Size (bp):
6528
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available

Vector Features:

Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
0
0
phage origin
f1
f1 phage origin
0
0
promoter
T7
T7 promoter
0
0
protease cleavage site
3C
3C protease cleavage site; separates the 6xHis tag and MBP from the insert
0
0
selectable marker
KanR
Kanamycin resistance gene
0
0
tag
His
N-terminal 6xHis tag
0
0
tag
MBP
N-terminal MBP
0
0
trxn termination sequence
T7 term
T7 transcription termination site
0
0