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Detailed Vector Information: pHM3C-LIC

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Vector Name: pHM3C-LIC
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Synonyms: None

pHM3C-LIC

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments: None
Size (bp): 6528
Empty Vector: EvNO00292962
Parent Vector: None
Properties: bacterial expression
Author Name: Center for Structures of Membrane Proteins
University of California San Francisco
Marta Pioletti
Publications: None
Vector Map:         Vector Sequence:
Sequence: Not Available

 



Vector Features:

Type Name Description Start Position End Position
ligation independent cloning LIC Ligation independent cloning site 0 0
phage origin f1 f1 phage origin 0 0
promoter T7 T7 promoter 0 0
protease cleavage site 3C 3C protease cleavage site; separates the 6xHis tag and MBP from the insert 0 0
selectable marker KanR Kanamycin resistance gene 0 0
tag His N-terminal 6xHis tag 0 0
tag MBP N-terminal MBP 0 0
trxn termination sequence T7 term T7 transcription termination site 0 0