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Detailed Vector Information: pVP68K


Vector Name: pVP68K
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Synonyms: ''


Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments: Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp): 6353
Empty Vector: EvNO00306669
Parent Vector: None
Properties: bacterial expression
Author Name: University of Wisconsin
Center for Eukaryotic Structural Genomics
Publications: PMID: 18988018
Title: Flexi vector cloning.

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 4150 5377
gene fragment Barnase Barnase 1448 1780
primer MBP forward MBP forward primer site 1271 1291
primer T7 term T7 terminator reverse primer 2599 2616
promoter T5 T5 promoter region 122 202
protease cleavage site 3C 3C protease cleavage site 1389 1412
repressor binding site LacI LacI coding region 2980 4071
selectable marker KanR Kanamycin resistance gene 5385 6179
selectable marker CmR chloramphenicol acetyltransferase gene 1885 2544
tag His N-terminal 8xHis tag 237 260
tag MBP N-terminal Maltose Binding Protein tag 267 1363
trxn termination sequence rrnB rrnB transcription terminator 2796 2892