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Detailed Vector Information: pMIS2.1mv


Vector Name: pMIS2.1mv
       DyNA Vector Map

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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning.
Comments: Misticated vector with N-terminal HIS-tag based on pET vector with mistic from Bacilus subtilis.
Size (bp): 7562
Empty Vector: EvNO00304415
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: Salk Institute
Mark Vega
Center for Structures of Membrane Proteins
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 2289 2309
bacterial origin ColE ColE bacterial origin 4020 4702
death cassette ccdB ccdB death casette. Empty vector requires survival strain for growth 1883 2092
phage origin f1 f1 origin 2495 2801
protease cleavage site Thrombin thrombin cleavage site 145 162
recombination site attR1 attR1 recombinational cloning site 556 572
recombination site attR2 attR2 recombinational cloning site 2243 2258
repressor protein gene LacI Lac repressor gene 6114 7130
selectable marker AmpR Ampicillin resistance gene 3263 3922
tag His N-terminal 8xHis tag 2310 2327
tag MISTIC N-terminal Mistic protein (bacilus subtilis) tag. Used to increase membrane protein expression 247 498
trxn termination sequence lac operator lac operator 40 62
trxn termination sequence T7 T7 transcription terminator 2395 2441