Vector Name:
pMIS2.1mv
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Gateway compatible bacterial expression destination vector with N-terminal 8xHis tag and Mistic (from B. subtilis) tag; contains ccdB death cassette that is removed after recombinational cloning; ampicillin resistance in bacteria; recombinational cloning.
Comments:
Misticated vector with N-terminal HIS-tag based on pET vector with mistic from Bacilus subtilis.
Size (bp):
7562
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name:
Salk Institute
Mark Vega
Center for Structures of Membrane Proteins
PSI
Mark Vega
Center for Structures of Membrane Proteins
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
2289
2309
bacterial origin
ColE
ColE bacterial origin
4020
4702
death cassette
ccdB
ccdB death casette. Empty vector requires survival strain for growth
1883
2092
phage origin
f1
f1 origin
2495
2801
protease cleavage site
Thrombin
thrombin cleavage site
145
162
recombination site
attR1
attR1 recombinational cloning site
556
572
recombination site
attR2
attR2 recombinational cloning site
2243
2258
repressor protein gene
LacI
Lac repressor gene
6114
7130
selectable marker
AmpR
Ampicillin resistance gene
3263
3922
tag
His
N-terminal 8xHis tag
2310
2327
tag
MISTIC
N-terminal Mistic protein (bacilus subtilis) tag. Used to increase membrane protein expression
247
498
trxn termination sequence
lac operator
lac operator
40
62
trxn termination sequence
T7
T7 transcription terminator
2395
2441
