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Detailed Vector Information: pHIS8

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Vector Name: pHIS8
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Synonyms: None

pHIS8

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning.
Comments: pHIS8 vector is based on pET28. Contains a stop codon.
Size (bp): 6992
Empty Vector: EvNO00304413
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Structures of Membrane Proteins
PSI
Salk Institute
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE origin 3450 4132
death cassette ccdB ccdB death cassette. Survival strain must be used for growth 1498 1707
promoter T7 T7 promoter 21 40
protease cleavage site Thrombin Thrombin cleavage site 144 161
recombination site attR1 attR1 recombinational cloning site 171 187
recombination site attR2 attR2 recombinational cloning site 1858 1873
repressor protein gene LacI Lac repressor gene 5544 6560
ribosome binding site RBS ribosomal binding site (start and end positions estimated) 105 114
selectable marker CmR chloramphenicol resistance 404 1060
selectable marker KanR Kanamycin resistance marker 2592 3404
tag His N-terminal 8x His tag 114 137
trxn regulatory element lac operator lac operator 40 62
trxn termination sequence T7 term T7 transcriptional terminator 1957 2003