Vector Name:
pHIS8
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Gateway compatible bacterial expression vector with T7 promoter, N- and C-terminal His tags and thrombin cleavage site; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning.
Comments:
pHIS8 vector is based on pET28. Contains a stop codon.
Size (bp):
6992
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Salk Institute
Center for Structures of Membrane Proteins
PSI
Center for Structures of Membrane Proteins
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE origin
3450
4132
death cassette
ccdB
ccdB death cassette. Survival strain must be used for growth
1498
1707
promoter
T7
T7 promoter
21
40
protease cleavage site
Thrombin
Thrombin cleavage site
144
161
recombination site
attR1
attR1 recombinational cloning site
171
187
recombination site
attR2
attR2 recombinational cloning site
1858
1873
repressor protein gene
LacI
Lac repressor gene
5544
6560
ribosome binding site
RBS
ribosomal binding site (start and end positions estimated)
105
114
selectable marker
CmR
chloramphenicol resistance
404
1060
selectable marker
KanR
Kanamycin resistance marker
2592
3404
tag
His
N-terminal 8x His tag
114
137
trxn regulatory element
lac operator
lac operator
40
62
trxn termination sequence
T7 term
T7 transcriptional terminator
1957
2003
