Detailed Vector Information: pHIS9

Vector Name:
pHIS9
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Gateway compatible bacterial expression vector with T7 promoter and N-terminal His tag; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning.
Comments:
pHIS9 vector is based on pET28 with N-terminal HIS-tag and no stop codon
Size (bp):
6990
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Salk Institute
Center for Structures of Membrane Proteins
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE
ColE bacterial origin
3448
4130
death cassette
ccdB
ccdB death cassette. survival cell line required for growth
1483
1692
promoter
T7
T7 promoter
21
40
recombination site
attR1
attR1 recombinational cloning site
156
172
recombination site
attR2
attR2 recombinational cloning site
1843
1858
repressor protein gene
LacI
Lac repressor gene
5542
6558
selectable marker
CmR
Chloramphenicol resistance gene
389
1045
selectable marker
KanR
Kanamycin resistance
2590
3402
tag
His
N-terminal 9xHis tag
114
140
trxn regulatory element
lac operator
lac operator
40
62
trxn termination sequence
T7 term
T7 terminator sequence
1955
2001