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Detailed Vector Information: pHIS9

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Vector Name: pHIS9
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Synonyms: None

pHIS9

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Gateway compatible bacterial expression vector with T7 promoter and N-terminal His tag; contains ccdB death cassette that is removed after recombinational cloning; kanamycin resistance in bacteria; recombinational cloning.
Comments: pHIS9 vector is based on pET28 with N-terminal HIS-tag and no stop codon
Size (bp): 6990
Empty Vector: EvNO00304414
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Center for Structures of Membrane Proteins
PSI
Salk Institute
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE bacterial origin 3448 4130
death cassette ccdB ccdB death cassette. survival cell line required for growth 1483 1692
promoter T7 T7 promoter 21 40
recombination site attR1 attR1 recombinational cloning site 156 172
recombination site attR2 attR2 recombinational cloning site 1843 1858
repressor protein gene LacI Lac repressor gene 5542 6558
selectable marker CmR Chloramphenicol resistance gene 389 1045
selectable marker KanR Kanamycin resistance 2590 3402
tag His N-terminal 9xHis tag 114 140
trxn regulatory element lac operator lac operator 40 62
trxn termination sequence T7 term T7 terminator sequence 1955 2001