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Detailed Vector Information: pSGP46

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Vector Name: pSGP46
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Synonyms: None

pSGP46

Sequencing Primer: Forward:  pSGP-Forward
Reverse:  pSGP-Reverse
Description: Yeast expression vector with Adh2 promoter, adds C-terminal 3C-ZZ-10XHis tag; ampicillin resistance (bacteria); uracil resistance (yeast); ligation independent cloning (LIC).
Comments: S. cerevisiae expression strain
Size (bp): 6675
Empty Vector: EvNO00304416
Parent Vector: None
Properties: ligation independent cloning (LIC), with tag/fusion/marker, yeast expression
Author Name: Center for High Throughput Structural Biology
Mark Dumont
Kathleen Clark
PSI
Publications: PMID: 20045057
Title: Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ligation independent cloning LIC Ligation independent cloning site 0 0
promoter Ura3 promoter Ura3 promoter 6360 6389
protease cleavage site 3C Rhinovirus 3C protease cleavage site 4233 4256
selectable marker AmpR Ampicillin resistance gene 1742 2401
selectable marker Ura3 Ura3 nutritional marker (yeast) 5471 6274
tag His 10x His tag 4653 4682