Detailed Vector Information: pMA510

Vector Name:
pMA510
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).  
Comments:
Requires special host strain E. coli DB3.1 for growth
Size (bp):
7141
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
David Eisenberg
Mark Arbing
Integrated Center for Structure and Function Innovation
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial operon
lac operator
Lac operator CDS
5003
5025
bacterial origin
ColE
ColE1 pBR322 origin
1421
2103
death cassette
ccdB
ccdB death cassette
6637
3846
phage origin
f1
f1 phage origin
29
467
primer
T7 term primer
T7 terminator reverse sequencing primer
7058
7076
promoter
T7
T7 promoter
4984
5000
protease cleavage site
TEV
TEV protease cleavage site
6208
6228
repressor protein gene
LacI
LacI repressor gene
3515
4594
ribosome binding site
RBS
ribosomal binding site
5055
5063
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
MBP
N-termal MBP fusion tag
5071
6183
tag
His
N-terminal 6xHis tag
6190
6207