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Detailed Vector Information: pMA510


Vector Name: pMA510
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Synonyms: ''


Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with a T7 promoter, N-terminal MBP fusion-6xHis tag-TEV protease site; contains the ccdB death cassette; kanamycin resistance in bacteria; ligation independent cloning (LIC).  
Comments: Requires special host strain E. coli DB3.1 for growth
Size (bp): 7141
Empty Vector: EvNO00305687
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: David Eisenberg
Mark Arbing
Integrated Center for Structure and Function Innovation
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial operon lac operator Lac operator CDS 5003 5025
bacterial origin ColE ColE1 pBR322 origin 1421 2103
death cassette ccdB ccdB death cassette 6637 3846
phage origin f1 f1 phage origin 29 467
primer T7 term primer T7 terminator reverse sequencing primer 7058 7076
promoter T7 T7 promoter 4984 5000
protease cleavage site TEV TEV protease cleavage site 6208 6228
repressor protein gene LacI LacI repressor gene 3515 4594
ribosome binding site RBS ribosomal binding site 5055 5063
selectable marker KanR kanamycin resistance gene 563 1375
tag MBP N-termal MBP fusion tag 5071 6183
tag His N-terminal 6xHis tag 6190 6207