Detailed Vector Information: pMCSG28

Vector Name:
pMCSG28
Synonyms:
''
Sequencing Primer:
Forward:  T7
Reverse:  T7 terminator
Description:
Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments:
For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp):
5253
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI
Argonne National Laboratory
Publications:
PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR332 origin of replication
3247
3247
primer
T7 forward
T7 forward sequencing primer
324
343
primer
T7 reverse
T7 terminator reverse sequencing primer
66
84
promoter
T7
T7 promoter (on complementary strand)
327
343
protease cleavage site
TEV
TEV protease cleavage site (on complementary strand)
232
252
recombination site
LIC
LIC Site (on complementary strand)
245
265
repressor binding site
lac operator
LacO fragment
302
324
repressor protein gene
LacI
LacI regulatory gene
857
1816
selectable marker
AmpR
Ampicillin resistance
4005
4865
tag
His
6xHis tag (on complementary strand)
208
225
trxn termination sequence
T7 term
T7 terminator (on complementary strand)
26
72