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Detailed Vector Information: pMCSG28

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Vector Name: pMCSG28
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Synonyms: None

pMCSG28

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and TEV-cleavage C-terminal 6xHis; ampicillin resistance in bacterial; ligation independent cloning (LIC)
Comments: For more information about MCSG vectors visit their website at: http://bioinformatics.anl.gov/mcsg/technologies/vectors.html
Size (bp): 5253
Empty Vector: EvNO00340964
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI
Midwest Center for Structural Genomics
Argonne National Laboratory
Publications: PMID: 20213425
Title: Cleavable C-terminal His-tag vectors for structure determination.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR332 origin of replication 3247 3247
primer T7 reverse T7 terminator reverse sequencing primer 66 84
primer T7 forward T7 forward sequencing primer 324 343
promoter T7 T7 promoter (on complementary strand) 327 343
protease cleavage site TEV TEV protease cleavage site (on complementary strand) 232 252
recombination site LIC LIC Site (on complementary strand) 245 265
repressor binding site lac operator LacO fragment 302 324
repressor protein gene LacI LacI regulatory gene 857 1816
selectable marker AmpR Ampicillin resistance 4005 4865
tag His 6xHis tag (on complementary strand) 208 225
trxn termination sequence T7 term T7 terminator (on complementary strand) 26 72