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Detailed Vector Information: pMYC4-I


Vector Name: pMYC4-I
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Synonyms: ''


Sequencing Primer: Forward:  Ps-Pimyc
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments: Target gene controlled by Pimyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp): 4936
Empty Vector: EvNO00306567
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 216 342
bacterial origin ColE ColE1 replication origin (on complementary strand) 1021 1555
mycobacterial origin myc ori mycobacterial origin of replication 1742 3587
promoter Pimyc Pimyc promoter 85 215
selectable marker HygR hygromycin resistance cassette 3924 4922
tag His C-terminal His tag 279 311
trxn termination sequence rrnB rrnB T2 transcriptional terminator 50 70
trxn termination sequence T4g32 T4g32 transcription terminator 357 377