Detailed Vector Information: pMYC4-I

Vector Name:
pMYC4-I
Synonyms:
''
Sequencing Primer:
Forward:  Ps-Pimyc
Reverse:  Unknown
Description:
Mycoplasma coexpression expression vector with the Pimyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments:
Target gene controlled by Pimyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp):
4936
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
216
342
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
1021
1555
mycobacterial origin
myc ori
mycobacterial origin of replication
1742
3587
promoter
Pimyc
Pimyc promoter
85
215
selectable marker
HygR
hygromycin resistance cassette
3924
4922
tag
His
C-terminal His tag
279
311
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
50
70
trxn termination sequence
T4g32
T4g32 transcription terminator
357
377