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Detailed Vector Information: pMYC4-S


Vector Name: pMYC4-S
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Synonyms: ''


Sequencing Primer: Forward:  Ps-Pimyc
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments: Target gene controlled by Psmyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp): 5115
Empty Vector: EvNO00306568
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 395 521
bacterial origin ColE ColE1 replication origin (on complementary strand) 1200 1734
mycobacterial origin myc ori mycobacterial origin of replication 1321 3766
promoter Psmyc Psmyc promoter 84 393
selectable marker HygR hygromycin resistance cassette 4103 5101
tag His His tag 458 490
tag myc C-terminal c-myc tag 458 493
trxn termination sequence rrnB rrnB T2 transcriptional terminator 49 69
trxn termination sequence T4g32 T4g32 transcription terminator 536 556