Vector Name:
pMYC4-Hp
Synonyms:
''
Sequencing Primer:
Forward:
Phsp
Reverse:
Unknown
Description:
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments:
Target gene controlled by Phsp promoter for expression is cloned between PacI and SbfI restriction sites. This is the newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites.
Size (bp):
5185
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID:
19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
Multiple cloning site
465
591
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
1270
1804
mycobacterial origin
myc ori
mycobacterial origin of replication
1991
3836
promoter
Phsp
Phsp promoter
85
464
selectable marker
HygR
hygromycin resistance cassette
4173
5171
tag
His
His tag
528
560
tag
myc
C-terminal c-myc tag
528
563
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
50
70
trxn termination sequence
T4g32
T4g32 transcription terminator
606
626
