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Detailed Vector Information: pMYC4-Hp


Vector Name: pMYC4-Hp
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Synonyms: ''


Sequencing Primer: Forward:  Phsp
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PacI, SbfI).
Comments: Target gene controlled by Phsp promoter for expression is cloned between PacI and SbfI restriction sites. This is the newer version of pMYC4-H suitable for universal cloning by PacI and SbfI sites.
Size (bp): 5185
Empty Vector: EvNO00306569
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS Multiple cloning site 465 591
bacterial origin ColE ColE1 replication origin (on complementary strand) 1270 1804
mycobacterial origin myc ori mycobacterial origin of replication 1991 3836
promoter Phsp Phsp promoter 85 464
selectable marker HygR hygromycin resistance cassette 4173 5171
tag His His tag 528 560
tag myc C-terminal c-myc tag 528 563
trxn termination sequence rrnB rrnB T2 transcriptional terminator 50 70
trxn termination sequence T4g32 T4g32 transcription terminator 606 626