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Detailed Vector Information: p4-Hb


Vector Name: p4-Hb
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Synonyms: ''


Sequencing Primer: Forward:  Phsp
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
Comments: Target gene controlled by Phsp promoter for expression is cloned between AsiSI and PmeI restriction sites.
Size (bp): 5177
Empty Vector: EvNO00306570
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS Multiple cloning site 84 583
bacterial origin ColE ColE1 replication origin (on complementary strand) 1262 1796
mycobacterial origin myc ori mycobacterial origin of replication 1983 3828
promoter Phsp Phsp promoter 84 468
selectable marker HygR hygromycin resistance cassette 4165 5163
tag His His tag 520 552
tag myc C-terminal c-myc tag 520 555
trxn termination sequence rrnB rrnB T2 transcriptional terminator 49 69
trxn termination sequence T4g32 T4g32 transcription terminator 598 618