Detailed Vector Information: p4-Hb

Vector Name:
p4-Hb
Synonyms:
''
Sequencing Primer:
Forward:  Phsp
Reverse:  Unknown
Description:
Mycoplasma coexpression expression vector with the Phsp promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
Comments:
Target gene controlled by Phsp promoter for expression is cloned between AsiSI and PmeI restriction sites.
Size (bp):
5177
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:

Vector Features:

Type
Name
Description
Start
End
MCS
MCS
Multiple cloning site
84
583
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
1262
1796
mycobacterial origin
myc ori
mycobacterial origin of replication
1983
3828
promoter
Phsp
Phsp promoter
84
468
selectable marker
HygR
hygromycin resistance cassette
4165
5163
tag
His
His tag
520
552
tag
myc
C-terminal c-myc tag
520
555
trxn termination sequence
rrnB
rrnB T2 transcriptional terminator
49
69
trxn termination sequence
T4g32
T4g32 transcription terminator
598
618