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Detailed Vector Information: p4-Sb


Vector Name: p4-Sb
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Synonyms: None


Sequencing Primer: Forward:  p4
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the Psmyc promoter and C-termial c-myc tag; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (AsiSI, PmeI).
Comments: Target gene controlled by Psmyc promoter for expression is cloned between AsiSI and PmeI restriction sites.
Size (bp): 5108
Empty Vector: EvNO00306572
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 84 398
bacterial origin ColE ColE1 replication origin (on complementary strand) 1193 1727
mycobacterial origin myc ori mycobacterial origin of replication 1914 3759
promoter Psmyc Psmyc promoter 84 398
selectable marker HygR hygromycin resistance cassette 4096 5094
tag His His tag 451 483
tag myc C-terminal c-myc tag 451 486
trxn termination sequence T4g32 T4g32 transcription terminator 529 549
trxn termination sequence rrnB rrnB T2 transcriptional terminator 49 69