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Detailed Vector Information: p9T-c


Vector Name: p9T-c
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Synonyms: ''


Sequencing Primer: Forward:  PtetO
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI).
Comments: Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second constitutive expression cassette with another target gene originally cloned into the intermediate vector p4-Sb, p4-Hb or p4-Ib is transferred to this vector between PacI and SbfI restriction sites.
Size (bp): 8203
Empty Vector: EvNO00306573
Parent Vector: None
Properties: multiple cloning site, with tag/fusion/marker
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 376 418
bacterial origin ColE ColE1 replication origin (on complementary strand) 6361 6894
mycobacterial origin myc ori mycobacterial origin of replication 1048 3522
promoter PtetO UV15 with two tet operators; tetracycline-inducible promoter 65 370
promoter Psmyc Psmyc promoter 3784 4190
repressor protein gene TET tetR(B) Tet Repressor class B 4217 4840
selectable marker HygR hygromycin resistance cassette (on complementary strand) 4947 5945
trxn termination sequence rrnB 2 rrnBT2 transcriptional terminators; located from nucleotides 24-43 and 3834-3853 24 43
trxn termination sequence T4g32 T4g32 transcription terminator 939 957