Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI).
Comments:
Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second constitutive expression cassette with another target gene originally cloned into the intermediate vector p4-Sb, p4-Hb or p4-Ib is transferred to this vector between PacI and SbfI restriction sites.
Size (bp):
8203
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI