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Detailed Vector Information: p9T-H


Vector Name: p9T-H
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Synonyms: ''


Sequencing Primer: Forward:  Phsp
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the PtetO promoter and Phsp promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Phsp gene: PacI, SbfI).
Comments: Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Phsp promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp): 8203
Empty Vector: EvNO00306585
Parent Vector: None
Properties: multiple cloning site
Author Name: Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS Multiple cloning site 376 412
bacterial origin ColE ColE1 replication origin (on complementary strand) 6803 7336
mycobacterial origin myc ori mycobacterial origin of replication 1490 3964
promoter PtetO UV15 with two tet operators; tetracycline-inducible promoter 72 370
promoter Psmyc Psmyc promoter 4316 4632
promoter Phsp Phsp promoter 962 1333
repressor binding site TET tetR(B) Tet Repressor class B 4659 5282
selectable marker HygR hygromycin resistance cassette (on complementary strand) 5389 6387
trxn termination sequence rrnB 3 rrnBT2 transcriptional terminators; located from nucleotides 24-43, 913-932 and 4276-4295 24 43
trxn termination sequence T4g32 2 T4g32 transcription terminator; located from nucleotides 1381-1399 and 5318-5336 1381 1399