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Detailed Vector Information: p9T-I


Vector Name: p9T-I
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Synonyms: None


Sequencing Primer: Forward:  PtetO
Reverse:  Unknown
Description: Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI).
Comments: Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Pimyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp): 8203
Empty Vector: EvNO00306605
Parent Vector: None
Properties: multiple cloning site
Author Name: Center for Eukaryotic Structural Genomics
Brian Fox
Phil Brumm
Yong Chang
David Mead
Vinay Dhodda
Publications: PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies

Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
MCS MCS multiple cloning site 376 412
bacterial origin ColE ColE1 replication origin (on complementary strand) 6554 7087
gene fragment 83170 C. merolae 10D unknown heme-binding protiein; used as a 3' stablibier of gene expression 427 879
mycobacterial origin myc ori mycobacterial origin of replication 1241 3715
promoter Pimyc Pimyc promoter 962 1084
promoter PtetO UV15 with two tet operators; tetracycline-inducible promoter 72 370
promoter Psmyc Psmyc promoter 4067 4383
repressor protein gene TET tetR(B) Tet Repressor class B 4410 5033
selectable marker HygR hygromycin resistance cassette (on complementary strand) 5140 6138
trxn termination sequence T4g32 2 T4g32 transcription terminator; located from nucleotides 1132-1150 and 5069-5087 1132 1150
trxn termination sequence rrnB 3 rrnBT2 transcriptional terminators; located from nucleotides 24-43, 913-932 and 4027-4046 24 43