Detailed Vector Information: p9T-I

Vector Name:
p9T-I
Synonyms:
''
Sequencing Primer:
Forward:  PtetO
Reverse:  Unknown
Description:
Mycoplasma coexpression expression vector with the PtetO promoter and Pimyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Pimyc gene: PacI, SbfI).
Comments:
Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Pimyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp):
8203
Parent Vector:
None
Properties:
multiple cloning site
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
376
412
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
6554
7087
gene fragment
83170
C. merolae 10D unknown heme-binding protiein; used as a 3' stablibier of gene expression
427
879
mycobacterial origin
myc ori
mycobacterial origin of replication
1241
3715
promoter
PtetO
UV15 with two tet operators; tetracycline-inducible promoter
72
370
promoter
Psmyc
Psmyc promoter
4067
4383
promoter
Pimyc
Pimyc promoter
962
1084
repressor protein gene
TET
tetR(B) Tet Repressor class B
4410
5033
selectable marker
HygR
hygromycin resistance cassette (on complementary strand)
5140
6138
trxn termination sequence
rrnB
3 rrnBT2 transcriptional terminators; located from nucleotides 24-43, 913-932 and 4027-4046
24
43
trxn termination sequence
T4g32
2 T4g32 transcription terminator; located from nucleotides 1132-1150 and 5069-5087
1132
1150