Detailed Vector Information: p9T-S

Vector Name:
p9T-S
Synonyms:
''
Sequencing Primer:
Forward:  PtetO
Reverse:  Unknown
Description:
Mycoplasma coexpression expression vector with the PtetO promoter and Psmyc promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; Psmyc gene: PacI, SbfI).
Comments:
Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second gene controlled by Psmyc promoter for expression is cloned between PacI and SbfI restriction sites.
Size (bp):
8203
Parent Vector:
None
Properties:
multiple cloning site
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
376
412
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
6734
7267
gene fragment
83170
C. merolae 10D unknown heme-binding protiein; used as a 3' stablibier of gene expression
427
879
mycobacterial origin
myc ori
mycobacterial origin of replication
1421
3895
promoter
PtetO
UV15 with two tet operators; tetracycline-inducible promoter
72
370
promoter
Psmyc
Psmyc promoters; located a nucleotides 4247-4563 and 692-1264
4247
4563
repressor protein gene
TET
tetR(B) Tet Repressor class B
4590
5213
selectable marker
HygR
hygromycin resistance cassette (on complementary strand)
5320
6318
trxn termination sequence
rrnB
3 rrnBT2 transcriptional terminators; located from nucleotides 24-43, 913-932 and 4207-4226
24
43
trxn termination sequence
T4g32
2 T4g32 transcription terminator; located from nucleotdies 1312-1330 and5249-5267
1312
1330