Vector Name:
pVP80K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial expression vector with a T5 promoter and N-terminal 8xHis-MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp):
7717
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
PSI
Center for Eukaryotic Structural Genomics
Center for Eukaryotic Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
5514
6741
gene fragment
sacB
sucrase gene from B. subtillis
1651
3075
gene fragment
CAT
chloramphenicol acetyltransferase gene
3249
3903
primer
MBP forward
MBP forward sequencing primer
1271
1291
primer
T7 term primer
T7 terminator reverse sequencing primer
3902
3920
promoter
T5
T5 promoter
122
202
protease cleavage site
3C
3C protease cleavage site
1389
1412
repressor protein gene
LacI
LacI coding region
4143
4162
selectable marker
KanR
kanamycin resistance cassette (on complementary strand)
6749
7543
tag
MBP
N-terminal MBP tag
267
1363
tag
His
N-terminal 8xHis tag
237
260
trxn termination sequence
T7 reverse
T7 terminator reverse (on complementary strand)
3963
3980
trxn termination sequence
rrnB
rrnB transcription termination
4160
4256
