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Detailed Vector Information: pVP80K


Vector Name: pVP80K
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Synonyms: ''


Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial expression vector with a T5 promoter and N-terminal 8xHis-MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments: Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp): 7717
Empty Vector: EvNO00306645
Parent Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: PSI
Center for Eukaryotic Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 5514 6741
gene fragment sacB sucrase gene from B. subtillis 1651 3075
gene fragment CAT chloramphenicol acetyltransferase gene 3249 3903
primer MBP forward MBP forward sequencing primer 1271 1291
primer T7 term primer T7 terminator reverse sequencing primer 3902 3920
promoter T5 T5 promoter 122 202
protease cleavage site 3C 3C protease cleavage site 1389 1412
repressor protein gene LacI LacI coding region 4143 4162
selectable marker KanR kanamycin resistance cassette (on complementary strand) 6749 7543
tag MBP N-terminal MBP tag 267 1363
tag His N-terminal 8xHis tag 237 260
trxn termination sequence T7 reverse T7 terminator reverse (on complementary strand) 3963 3980
trxn termination sequence rrnB rrnB transcription termination 4160 4256