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Detailed Vector Information: pVP81K


Vector Name: pVP81K
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Synonyms: ''


Sequencing Primer: Forward:  MBP Forward
Reverse:  T7 Terminator
Description: Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments: Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp): 8538
Empty Vector: EvNO00306665
Parent Vector: None
Properties: bacterial expression, with tag/fusion/marker
Author Name: Center for Eukaryotic Structural Genomics
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin of replication 6341 7568
gene fragment TVMV cds TVMV protease gene (on complementary strand) 139 849
gene fragment sacB sucrase gene from B. subtillis 2481 3902
gene fragment CAT chloramphenicol acetyltransferase gene 4074 4730
primer MBP forward MBP forward sequencing primer 2071 2091
promoter T5 T5 promoter 952 1032
promoter TET TET promoter (on complementary strand) 873 946
protease cleavage site TVMV TVMV protease cleavage site 2189 2209
repressor protein gene LacI LacI-wt coding region (on complementary strand) 5171 6262
selectable marker KanR kanamycin resistance cassette 7577 8538
tag MBP Auto-cleaving N-terminal MBP tag 1061 2165
tag His N-terminal 8xHis tag 2210 2234
trxn termination sequence T7 term T7 terminator reverse (on complementary strand) 4790 4807
trxn termination sequence rrnB rrnB transcription termination 15 111