Vector Name:
pVP81K
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
T7 Terminator
Description:
Bacterial expression vector with a T5 promoter with an auto-cleaving (TVMV protease) N-terminal MBP tag that leaves an N-terminal 8xHis tagged protein; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
Cloning into this vector will remove the sacB/CAT cassette and allow growth in the presence of 5% sucrose; empty vector should be proagated in dam- E. coli strain
Size (bp):
8538
Parent Vector:
None
Properties:
bacterial expression, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
PSI
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin of replication
6341
7568
gene fragment
TVMV cds
TVMV protease gene (on complementary strand)
139
849
gene fragment
sacB
sucrase gene from B. subtillis
2481
3902
gene fragment
CAT
chloramphenicol acetyltransferase gene
4074
4730
primer
MBP forward
MBP forward sequencing primer
2071
2091
promoter
T5
T5 promoter
952
1032
promoter
TET
TET promoter (on complementary strand)
873
946
protease cleavage site
TVMV
TVMV protease cleavage site
2189
2209
repressor protein gene
LacI
LacI-wt coding region (on complementary strand)
5171
6262
selectable marker
KanR
kanamycin resistance cassette
7577
8538
tag
MBP
Auto-cleaving N-terminal MBP tag
1061
2165
tag
His
N-terminal 8xHis tag
2210
2234
trxn termination sequence
T7 term
T7 terminator reverse (on complementary strand)
4790
4807
trxn termination sequence
rrnB
rrnB transcription termination
15
111
