Detailed Vector Information: pDONR223

Vector Name:
pDONR223
Synonyms:
''
Sequencing Primer:
Forward:  M13F
Reverse:  M13R
Description:
Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 bacterial origin
4339
4958
death cassette
ccdB
ccdB negative selection gene (death cassette), LOST in recombined (with insert) form
1200
1409
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
3020
3040
recombination site
attP1
attP recombination site 1 present in empty vector with death cassette, replaced by attL1 in recombined form with gene of interest or other insert
569
652
recombination site
attP2
attP recombination site 2 present in empty vector with death cassette, replaced by attL2 in recombined form with gene of interest or other insert
2896
2983
selectable marker
SpectR
spectinomycin resistance gene
3471
4262
trxn termination sequence
rrnB
rrnB T1 and T2 terminator
268
470