Detailed Vector Information: pDONR221

Vector Name:
pDONR221
Synonyms:
''
Sequencing Primer:
Forward:  U19
Reverse:  AttP2
Description:
Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
DNA Vector Map
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Vector Features:

Type
Name
Description
Start
End
bacterial origin
ColE1
ColE1 (pUC-type) origin of replication
4086
4759
negative selection marker
ccdB
ccdB negative selection gene (death cassette), LOST in recombined (with insert) form
1502
1197
primer site
U19 primer
517
535
primer site
Att P2 Primer
3026
3043
primer site
M13 forward primer
M13 Forward priming site
537
552
primer site
M13 reverse primer
M13 Reverse priming site
3027
3034
recombination site
attP1
attP recombination site 1 LOST in recombined (with insert) form
570
801
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
570
801
recombination site
attP2
attP recombination site 2 LOST in recombined (with insert) form
2985
2754
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2985
2754
selectable marker
CmR
chloramphenicol resistance gene, LOST in recombined (with insert) form
2506
1847
selectable marker
KanR
kanamycin resistance gene
3156
3965
trxn termination sequence
rrnB T2
rrnB T2 transcription termination sequence
295
268
trxn termination sequence
rrnB T1
rrnB T1 transcription termination sequence
470
427