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Detailed Vector Information: pET32-DEST

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Vector Name: pET32-DEST
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Synonyms: None

pET32-DEST

Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and N-terminal Trx, 6xHis and S-tags; Thrombin and enterokinase cleavage sites; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments: None
Size (bp): 7611
Empty Vector: EvNO00548204
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: University of Georgia
Kelley Moremen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
death cassette ccdB ccdB death cassette (removed when gene insert is present) 5931 7236
promoter T7 T7 promoter 5120 5136
protease cleavage site Thrombin Thrombin cleavage site 5583 5600
protease cleavage site Enterokinase Enterokinase cleavage site 5667 5681
recombination site attR1 recombination site 5693 5821
recombination site attR2 recombination site 7277 7401
repressor protein gene LacI Lac repressor 3647 4606
selectable marker AmpR ampicillin resistance gene 598 1458
selectable marker CmR chloramphenicol resistance gene (removed when gene insert is present) 5930 6589
tag TRX N-terminal TRX tag 5208 5534
tag S-tag N-terminal S tag 5535 5552
tag His N-terminal 6xHis tag 5556 5573
trxn regulatory element lac operator Lac operator 5139 5163
trxn termination sequence T7 term T7 terminator 7525 7586