Vector Name:
pGEc1-DEST
Synonyms:
pXLG-CtermHisStrep-DEST
Sequencing Primer:
Forward:
pGEc1-DEST F
Reverse:
pGEn1-DEST R
Description:
Mammalian expression vector with a CMV promoter and C-terminal 8xHis and Strep tags; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
http://glycoenzymes.ccrc.uga.edu/
Size (bp):
6126
Parent Vector:
None
Properties:
Gateway, acceptor (destination), mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
Col E1 bacterial origin
1137
1804
death cassette
ccdB
ccdB death cassette (removed in plasmids with insert)
4211
4516
intron
artificial intron
artificial intron
2828
2960
poly-A signal
BGH pA
bovine growth hormone (BGH) polyadenylation signal
5302
5516
promoter
CMV
CMV promoter
2208
2790
recombination site
3'-ITR
inverted terminal repeat sequence
5604
5733
recombination site
5'ITR
inverted terminal repeat sequence
1998
2127
recombination site
attR1
attR1 site
2974
3101
recombination site
attR2
attR2 site
4557
4681
selectable marker
AmpR
ampicillin resistance gene
129
989
selectable marker
CmR
Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)
3210
2859
tag
His
C-terminal 8xHis tag
4691
4714
tag
strep
strep tag
4718
4741
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
4754
5295
