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Detailed Vector Information: pCPD_nHalo_DC


Vector Name: pCPD_nHalo_DC
       DyNA Vector Map

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Synonyms: pCPDnHalo-TEV_DC


Sequencing Primer: Forward:  HaloseqF
Reverse:  pCPD6268_R
Description: Bacterial expression vector with a T7 promoter and TEV cleavable N-terminal Halo tag; ampicillin resistance in bacteria; Gateway cloning
Comments: One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp): 7800
Empty Vector: EvNO00413804
Parent Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Ji Qiu
Justin Saul
Publications: None
Vector Map:         Vector Sequence:


Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 replication origin 1344 2026
death cassette ccdB ccdB death cassette (removed in clones containing an insert) 5954 7608
death cassette 2 ccdB (BsrGI removed) ccdB death cassette with BsrGI restriction site removed 7163 7468
primer HaloseqF forward sequencing primer (5' aagcctgcctaactgcaa 3') 5769 5786
primer pCPD6268_R reverse sequencing primer (5' agccaactcagcttccttt 3') 7688 7706
promoter T7 T7 promoter 4911 4927
protease cleavage site TEV TEV protease cleavage site (Promega EDLYYFQS) 5902 5922
recombination site attR2 recombination site 7609 7633
recombination site attR1 recombination site 5929 5953
repressor protein gene LacI LacI repressor 3438 4397
ribosome binding site RBS pET ribosomal binding site 4984 4990
selectable marker AmpR ampicillin resistance 587 1246
selectable marker CmR chloremphenicol resistance (removed in clones containing an insert) 6162 6818
tag HALO Halo tag 4999 5889
trxn regulatory element lac operator lac operator 4930 4953
trxn termination sequence T7 term T7 transcription termination 7728 7775