Vector Name:
pCPD_nHalo_DC
Synonyms:
pCPDnHalo-TEV_DC
Sequencing Primer:
Forward:
HaloseqF
Reverse:
pCPD6268_R
Description:
Bacterial expression vector with a T7 promoter and TEV cleavable N-terminal Halo tag; ampicillin resistance in bacteria; Gateway cloning
Comments:
One in a series of pCPD vectors made by J. Saul for E. coli protein expression at CPD.
Size (bp):
7800
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Ji Qiu
Justin Saul
Center for Personalized Diagnostics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 replication origin
1344
2026
death cassette
ccdB
ccdB death cassette (removed in clones containing an insert)
5954
7608
death cassette 2
ccdB (BsrGI removed)
ccdB death cassette with BsrGI restriction site removed
7163
7468
primer
HaloseqF
forward sequencing primer (5' aagcctgcctaactgcaa 3')
5769
5786
primer
pCPD6268_R
reverse sequencing primer (5' agccaactcagcttccttt 3')
7688
7706
promoter
T7
T7 promoter
4911
4927
protease cleavage site
TEV
TEV protease cleavage site (Promega EDLYYFQS)
5902
5922
recombination site
attR1
recombination site
5929
5953
recombination site
attR2
recombination site
7609
7633
repressor protein gene
LacI
LacI repressor
3438
4397
ribosome binding site
RBS
pET ribosomal binding site
4984
4990
selectable marker
CmR
chloremphenicol resistance (removed in clones containing an insert)
6162
6818
selectable marker
AmpR
ampicillin resistance
587
1246
tag
HALO
Halo tag
4999
5889
trxn regulatory element
lac operator
lac operator
4930
4953
trxn termination sequence
T7 term
T7 transcription termination
7728
7775
